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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Microbiology | 15-20% | - Identification of pathogenic microorganisms - Specimen collection and culture techniques - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology |
| Topic 2: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis |
| Topic 3: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes |
| Topic 4: Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Transfusion reactions and adverse events - Antibody detection and identification - Blood group systems and antigens - Compatibility testing |
| Topic 5: Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes - Laboratory safety and regulatory compliance |
| Topic 6: Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing |
| Topic 7: Immunology and Serology | 5-10% | - Immune system principles and components - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders - Infectious disease serology |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Beer's law is based on the fact that absorbance is directly proportional to the concentration of a solution.
Therefore, stray light can alter the absorbance results in this type of assay.
Deviations from Beer's Law are caused by:
A) polychromatic light
B) stray light
C) very low concentration of absorbing material
D) very high concentrations of substance being measured in a colorimetric reaction
2. Each of the drugs/drug classes listed above have been known to cause drug-induced hemolytic anemia, although cephalosporins are the MOST COMMON cause. Cephalosporins can cause drug-induced hemolytic anemia when a patient produces antibodies to the particular cephalosporin drug in the presence of red blood cells. The drugs can alter the membrane appearance of the red blood cells, causing the body to mistake them as foreign. Complement becomes activated due to these antibodies; red cells are then destroyed causing hemolytic anemia. Dark urine, caused by intravascular hemolysis, is one of the most common symptoms associated with this condition.
Which one of the following drugs/drug classes is the MOST COMMON cause of drug-induced hemolytic anemia?
A) Cephalosporins
B) Levodopa
C) Nonsteroidal anti-inflammatory drugs (NSAIDs)
D) Levofloxacin
E) Quinidine
3. Fatty casts contain refractile oval fat bodies or free fat droplets. They are derived from renal tubular cells, and may be seen in nephrotic syndrome.
Identify the urine sediment element:
A) WBC cast
B) Waxy cast
C) Granular cast
D) Fatty cast
4. Which immune elements are involved in a POSITIVE TB skin test?
A) Epithelial skin cells
B) Nkcells and IgG antibodies
C) B cells and IgM antibodies
D) IgE T cells and macrophages
5. Where can one find guidance on the minimum performance standards for clinical laboratories?
A) Food and Drug Administration (FDA)
B) Clinical Laboratory Improvement Amendments of 1988 (CLIA '88)
C) National Institutes of Health
D) Centers for Disease Control and Prevention (CDC)
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: A | Question # 3 Answer: D | Question # 4 Answer: C | Question # 5 Answer: B |
