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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Immunology and Serology | 5-10% | - Immunologic testing methodologies - Infectious disease serology - Immune system principles and components - Autoimmune and immunodeficiency disorders |
| Topic 2: Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Topic 3: Microbiology | 15-20% | - Identification of pathogenic microorganisms - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques |
| Topic 4: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Semen and amniotic fluid testing - Urine formation and composition |
| Topic 5: Hematology | 20-25% | - Red blood cell physiology and disorders - White blood cell morphology and diseases - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Topic 6: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes |
| Topic 7: Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Blood components and storage - Blood group systems and antigens - Compatibility testing - Antibody detection and identification |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
Match each of the descriptions with the appropriate magnification:
1. Color, Rouleau, Overall Slide Quality, Cell Distribution
2. Platelet estimates RBC-platelet-WBC morphology WBC differential RBC inclusions
3. Select area to examine, WBC estimate
A. 10X
B. 100X (Oil)
C. 40X (Dry)
Question 2
A positive RPR test and a negative FTA-ABS test is most likely the result of:
A. Latent syphilis
B. Primary syphilis
C. False positive reaction
D. Secondary syphilis
Question 3
When making a platelet concentrate, the proper procedure is to start with a low centrifugation of the whole blood bag. After the plasma is removed, it is centrifuged again at a higher speed to separate the platelet portion from the plasma portion.
Blood bank
The following steps must be followed in preparation of a platelet concentrate:
A. Whole blood centrifuged at low speed - plasma separated then centrifuged at high speed
B. Whole blood centrifuged at high speed - plasma separated then centrifuged at high speed
C. Whole blood centrifuged at high speed - plasma separated then centrifuged at low speed
D. Whole blood centrifuged at low speed - plasma separated then centrifuged at low speed
Question 4
The conidia of Exophiala species are produced within phialides that extrude the conidia in tight, ball-like clusters.
The large, drum stick-shaped muriform macroconidia of Alternaria species are arranged in short chains; the small, elliptical conidia of Cladosporium species are arranged in both long and short, branching chains.
Which of the following dematiaceous fungal species produce conidia in clusters?
A. Alternaria species
B. Exophiala species
C. Cladosporium species
Question 5
Acute hemolytic transfusion reactions are most commonly due to ABO-incompatible blood being transfused to a recipient with naturally occurring ABO alloantibodies (anti-A, anti-B, anti A,B).
Acute intravascular hemolysis as the result of a blood transfusion is most often associated with which of the following causes?
A. Passively transfused antibodies to HLA antigens
B. Allergies
C. Transfusion of ABO incompatible red cells
D. Transfusion-associated graft-versus-host disease
Solutions:
| Question 1 Answer: A,B,C | Question 2 Answer: C | Question 3 Answer: A | Question 4 Answer: B | Question 5 Answer: C |
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